Summary: Coulter XL - FlowJo question
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From: Karim Vermaelen <kvermael@[EMAIL PROTECTED]
>
Date: Mon Feb 23 2004 - 12:17:48 EST
Thanks Mario, Adam, Kirk, Cris, Alessio and Jennifer for answering my
question about handling Coulter XL files in FlowJo. FlowJo 4 allows
you
to map any keyword to the data file column name:
(from Jennifer Wil****re:)
"You can use any FCS keyword as the name of the data file in the
FlowJo
workspace. In the Preferences, you have a choice of showing either:
1) the file's name according to the file name keyword (eg,
Z0015266.LMD)
2) the file's name according to the file name show in the finder
(sometimes
different from #1!)
3) any keyword or combination of keywords you choose (eg, $SRC)
This option is under the Workspace tab in the Preferences."
Unfortunately I have FlowJo v3.7.1!
Begin forwarded message:
> From: Karim Vermaelen <kvermael@[EMAIL PROTECTED]
>
> Date: February 13, 2004 11:21:08 CST
> To: Cytometry Mailing List <cytometry@[EMAIL PROTECTED]
>
> Subject: Coulter XL - FlowJo question
>
> Hi y'all,
> Currently I use FlowJo to do offline analysis of data acquired on a
> Coulter XL running "System II". I am more accustomed to BD equipment
> though. Files acquired on a BD system (Cellquest) have their sample
> description Files acquired on a BD system (Cellquest) under the "Title"
field in the FCS
> file. The contents of the "Title" field automatically appears under
> the sample's "Name" column in FlowJo.
> Files acquired under the XL's "System II" have their sample
> description recorded under the "#SRC" field. The "Title" field
> contains something not very useful such as "Z0015266.LMD" (ie same as
> the actual File name on the disk). I know you can add a "#SRC" column
> to your FlowJo workspace, but (1) a given sample name can be assigned
> to any #SRC you want, which could lead to errors, and (2) the titles
> of the FlowJo graph windows will display the XL's cryptic "Z...LMD"
> sample names anyway.
> Question:
> Is there any way to have the detailed sample description in Coulter XL
> files mapped to the FCS "Title" field instead of the "#SRC" header?
> ...that is without having to manually edit the FCS files one by one?
> Thanks for any help!
> Karim
>
>
>
> Karim Y. Vermaelen, MD, PhD
> Cellular and Molecular Biology Research Laboratory
> Building 37 - Room 1096
> NASA - Lyndon B. Johnson Space Center
> 2101 NASA Parkway
> Houston, Texas 77058
>
> tel (+281 24)4-1993
> fax (+281 48)3-3058
Received on Tue Feb 24 12:18:00 2004
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