Re: Summary: Coulter XL - FlowJo question
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From: Nick Haining <nicholas_haining@[EMAIL PROTECTED]
>
Date: Thu Feb 26 2004 - 12:47:39 EST
Being able to add experiment information into text fields associated
with an LMD file is very useful. We use RXP software with our
Coulter
FC500. Although there are SampleID fields that can be filled in at
the
time of acquisition, I've noticed when analyzing the files in Flowjo
there are a number of keyword fields that show up with the file
($CELLS: or $EXPERIMENT:, for example), that I don't seem to be able
to access in RXP software when acquiring. Anyone know how to make
RXP
have these fields show up in the acquisition window?
Thanks,
Nick Haining.
______________________
W. Nicholas Haining, B.M., B.Ch.
Pediatric Oncology/Hematology
Dana-Farber Cancer Institute
Children's Hospital
On Feb 23, 2004, at 12:17 PM, Karim Vermaelen wrote:
> Thanks Mario, Adam, Kirk, Cris, Alessio and Jennifer for answering my
> question about handling Coulter XL files in FlowJo. FlowJo 4 allows you
> to map any keyword to the data file column name:
>
> (from Jennifer Wil****re:)
>
> "You can use any FCS keyword as the name of the data file in the FlowJo
>
> workspace. In the Preferences, you have a choice of showing either:
>
> 1) the file's name according to the file name keyword (eg,
> Z0015266.LMD)
>
>
> 2) the file's name according to the file name show in the finder
> (sometimes
> different from #1!)
>
> 3) any keyword or combination of keywords you choose (eg, $SRC)
>
> This option is under the Workspace tab in the Preferences."
>
>
> Unfortunately I have FlowJo v3.7.1!
>
>
>
>
> Begin forwarded message:
>
>
>> From: Karim Vermaelen <kvermael@[EMAIL PROTECTED]
>
>> Date: February 13, 2004 11:21:08 CST
>> To: Cytometry Mailing List <cytometry@[EMAIL PROTECTED]
>
>> Subject: Coulter XL - FlowJo question
>>
>> Hi y'all,
>> Currently I use FlowJo to do offline analysis of data acquired on a
>> Coulter XL running "System II". I am more accustomed to BD equipment
>> though. Files acquired on a BD system (Cellquest) have their sample
>> description ("sample ID") recorded under the "Title" field in the FCS
>> file. The contents of the "Title" field automatically appears under
>> the sample's "Name" column in FlowJo.
>> Files acquired under the XL's "System II" have their sample
>> description recorded under the "#SRC" field. The "Title" field
>> contains something not very useful such as "Z0015266.LMD" (ie same as
>> the actual File name on the disk). I know you can add a "#SRC" column
>> to your FlowJo workspace, but (1) a given sample name can be assigned
>> to any #SRC you want, which could lead to errors, and (2) the titles
>> of the FlowJo graph windows will display the XL's cryptic "Z...LMD"
>> sample names anyway.
>> Question:
>> Is there any way to have the detailed sample description in Coulter XL
>> files mapped to the FCS "Title" field instead of the "#SRC" header?
>> .that is without having to manually edit the FCS files one by one?
>> Thanks for any help!
>> Karim
>>
>>
>>
>> Karim Y. Vermaelen, MD, PhD
>> Cellular and Molecular Biology Research Laboratory
>> Building 37 - Room 1096
>> NASA - Lyndon B. Johnson Space Center
>> 2101 NASA Parkway
>> Houston, Texas 77058
>>
>> tel (+281 24)4-1993
>> fax (+281 48)3-3058
>>
>>
Received on Fri Feb 27 14:58:00 2004
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