DiVa software problems
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From: Helen Ferry <helen.ferry@[EMAIL PROTECTED]
>
Date: Wed Jun 18 2008 - 13:21:47 EDT
Dear FLOWers,
We have been experiencing major problems with the DiVa software we use
on our LSRII. We also have some problems with our Aria that we would
like to know if anyone else has experienced, as we are not sure if
they
are related to the software or not.
We initially saw the problems using DiVa v5.0 and hoped that the new
DiVa v6.1.1. would fix the problems, but it hasn=E2=80=99t.
LSRII
1. During acquisition some or all of the dot plots are empty. When the
sample acquisition is complete, and the file has been saved, then the
data is displayed as normal. Obviously this is far from ideal since
you
need to be able to see if your sample during acquisition in case there
are any problems.
2. Some people have also run a set of samples and then gone back to
find
that the saved files are no longer there.
3. Since DiVa v6.1.1 was installed, they have also seen that the
machine
does not stop acquiring when it should.
ARIA
The software fails to respond when we click on unload, acquire, sort
etc. The only way out of this, is to force quit and restart the
software. We have wasted huge amounts of time during sorts messing
about
with this, as you have to wait for the stream to stabilize etc. We
have
also lost some samples because even though you have clicked to stop
acquiring, it doesn=E2=80=99t always actually stop.
Has anyone experienced any of the same problems and if so, how did you
fix it? Is it possible that it is related to the number of experiments
in the DiVa software, I know there is a limit to how much data you can
acquire into a single =E2=80=98Experiment=E2=80=99, but does the number of
separate
experiments affect the performance of the software?
Any help/input will be hugely appreciated.
Best wishes
Helen
Helen Ferry, D.Phil
FACS Manager
Haemopoietic Stem Cell Laboratory
Weatherall Institute for Molecular Medicine
John Radcliffe Hospital
University of Oxford
Headington
Oxford
OX3 9DS
United Kingdom
+44 1865 222340
Received on Thu Jun 19 13:18:00 2008
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