RE: DiVa nozzle salt volcano
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From: Ray Hicks <rhicks@[EMAIL PROTECTED]
>
Date: Mon Jun 23 2008 - 17:32:04 EDT
Hi Frank,
I saw this from time to time, it didn=E2=80=99t seem to correlate with
nozzle or buffer mix (home made or premixed pbs) =E2=80=93 maybe it=E2=80=
=99s a
dry atmosphere, I understand you have those down there sometimes but I
guess you=E2=80=99d have had the problem before now :)
Ray
Ray Hicks
Cytek Development Inc
http://www.cytekdev.com
tel: +44 (0)208 1337 968 (UK/Europe)
fax: +44(0)208 5889 004
skype: ray.hicks.cytek
From: WEHICytometry [mailto:facs_copy@[EMAIL PROTECTED]
23 June 2008 04:01
To: cyto-inbox
Subject: DiVa nozzle salt volcano
In 31 years of flow cytometry I hadn't seen this before (although I
don't get out much). Lately we experience this phenomenon on our
FACSVantageSEDiVa where, in the course of a few hours, the nozzle
grows a salt volcano around the stream (I've attached a picture). This
does not affect the breakoff nor the sort streams nor even the
acquired data until the volcano grows high enough to impinge on the
first laser. It can also be removed easily by a wipe with a wet swab
(although that's really inconvenient if the need arises during a
sort). Note: we are talking about a 70 micron nozzle running PBS as
sheath at 30 psi - nothing fancy.
The question is: what causes it? We *see* no leaks outside the nozzle
tip but I'm wondering if the orifice has eroded and that's causing
seepage. Is there anyone who has seen this and/or has an idea of the
cause?
Frank Battye.
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Received on Tue Jun 24 15:18:00 2008
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