Re: 2N G2/M vs. 4N G0
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From: VSH Tech Sup****t <Tech@[EMAIL PROTECTED]
>
Date: Wed May 02 2007 - 16:39:14 EDT
Kerry
In your posting you refer to 2N cells that are in G2/M phase and 4N
cells in
G0. We typically refer to the 2N (or 2C) in G2/M as DNA Diploid G2/M
and
the 4N (or 4C) events as DNA Tetraploid G0G1 events.
There is no direct way to tell the difference between a DNA Diploid
G2M
event and a DNA Tetraploid G0G1 events in single-parameter DNA
analysis.
They both produce the same fluorescence signal which is counted in the
same
channel of the cytometer.
Your observation that to call something "DNA Tetraploid" requires
identifying other parts of the " DNA Tetraploid" population is a good
one.
We have established a rule set for evaluating DNA cell cycle
histograms and
the presence of 6C and 8C peaks is one of the decisions points. I
would be
happy to send you a copy of the rule set if you are interested.
Best regards,
Don
Donald J. Herbert
Technical Sup****t Manager
Verity Software House
Received on Thu May 3 14:38:00 2007
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